Welcome to Gene Target Solutions, Australian Distributor of Biotium GelRed.
GelRed™ is a next-generation fluorescent nucleic acid gel stain designed to replace the highly toxic ethidium bromide (EtBr). Developed by scientists at Biotium, GelRed™ is superior to EtBr and other EtBr alternatives by having a combination of low toxicity, high sensitivity and exceptional stability. It is a direct replacement for ETBr due to its almost identical spectrum.
Figure 1: Excitation (left) and emission (right) spectra of GelRed™ bound to dsDNA in TBE.
Why use GelRedTM?
Safter than Ethidium Bromide.
GelRed™ has been shown to be non-mutagenic and non-cytotoxic at concentrations well above the working concentrations used in gel staining .
EtBr has been the predominant dye used for nucleic acid gel staining for decades because of its low price and generally sufficient sensitivity. However, EtBr is a highly mutagenic chemical. The safety hazard and costs associated with decontamination and waste disposal can ultimately make the dye expensive and inconvenient to use.
GelRed™ avoids the toxicity of Ethidium Bromide because its dyes are unable to cross cell membranes, and therefore don’t interact with intracellular DNA. GelRed™ is also impenetrable to latex gloves.
Figure 2. GelRed™ gel stain is safer because it cannot penetrate cell membranes to bind DNA in living cells. HeLa cells were incubated at 37°C with 1X SYBR® Safe, or GelRed™, respectively. Images were taken following incubation with dye for 30 min using Cy®3 filter set for GelRed™. SYBR® Safe rapidly entered cells and stained nuclei. GelRed™ was unable to cross cell membranes, demonstrated by the absence of fluorescence staining.
Ultra Sensitive.
GelRed™ is more sensitive than EtBr, and ethidium bromide alternative SYBR® Safe.
GelRed™ and EtBR have virtually the same spectra (Figure 3) so you can directly replace EtBr with GelRed without changing your existing imaging system.
Figure 3: Comparison of GelRed™ and ethidium bromide (EtBr) in precast gel staining using 1% agarose gel in TBE buffer. Twofold serial dilutions of 1kb Plus DNA Ladder from Invitrogen were loaded onto each gel in 4 lanes in the amounts of 200ng, 100ng, 50ng and 25ng, respectively from left to right.
Extremely Stable
Another major advantage of GelRed™ DNA stain is its remarkable stability. GelRed is stable in solution at room temperature for long-term storage. You can store and handle GelRed the same way you do with EtBr.
Simple & Flexible to Use
Use GelRed in either precast or post electrophoresis gel staining, compatible with common downstream applications like cloning and sequencing .GelRed is sold as a solution in both DMSO and in water.
Compatible with standard UV transilluminator or visible light gel reader.
GelRed™ replaces EtBr with no optical setting change.
Compatible with downstream applications.
Use your usual gel extraction kit to remove dyes from DNA for cloning or sequencing.
Easy Disposal
GelRed passed environmental safety tests for direct disposal down the drain, or in regular rubbish. It is non-toxic to aquatic life.
GelRed™ can be purchased in Australia from:
Unit 3C, Building 4, 256b New Line Rd
DURAL NSW 2158
Ph: (02) 8090 2675
info@genetargetsolutions.com
www.genetargetsolutions.com.au
GelRedTM and its uses are covered by US patent numbers 796048, 7803943, and 8232050.
Materials from Biotium are sold for research use only, and are not intended for food, drug, household or cosmetic use.
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